
This bulletin summarizes the results of 1993-1996 yield
performance tests of alfalfa variety entries in Indiana.
This information, protected by copyright by the Purdue
Research Foundation, is presented under authority granted the
Indiana Agricultural Research Programs to conduct performance
trials, including interpretation of the data to the public,
and does not imply endorsement or recommendation by Purdue
University. Permission is granted to reproduce the tables
only in their entirety provided the source is referenced and
the data are not rearranged, manipulated, or reinterpreted. A
conspicuous disclaimer which states "endorsement or
recommendation by Purdue University is not implied" must
accompany any information reproduced. Additional copies of
this and other informative publications are available to
Indiana residents from their local Purdue Cooperative
Extension Service Office or by writing:
Additional copies of this publication are available to
Indiana residents from their local Purdue Cooperative
Extension Offices or by writing:
Media Distribution Center
301 South 2nd Street
Lafayette, IN 47905-1092
This document can also be accessed electronically by
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and in the body of the message type:
send acsonline B-739 

Acknowledgements
We appreciate the help of the personnel of the regional
Purdue Agricultural Centers, the Agronomy Research Center,
and the many student and temporary workers that have assisted
in these studies. We would like to thank the National Weather
Service for the data used in Appendix Figure 1 and Appendix
Table 2. We would also like to thank the Indiana Agricultural
Statistics Service for the information included in Appendix
Table 1 of this bulletin. A special thanks goes to Lou Jones,
our departmental artist. Much of the artwork that brightens
our bulletin is from her pen.

Rain fell frequently in 1993 as the state's first harvest
approached, delaying harvest at Columbia City until June 10.
In order to maintain a reasonable harvest schedule, watching
for breaks in the weather became a high priority. While
moisture and forage growth were plentiful in 1993, hay drying
conditions were poor for most of the year. Quality of much
hay suffered from rain damage as time frames of more than a
few days of sunshine were rare.
The winter of 1993-1994 was especially hard on alfalfa
stands in the southern part of the state. Stands were reduced
by half due to heaving and winter injury. Drought stress
worsened yields throughout the summer at Butlerville. Pockets
of drought plagued much of the state throughout the season,
and lowered Indiana hay supplies. First harvest was taken at
a mid-bud stage during a week of exceptionally good harvest
weather.
Early season rain in 1995 resulted in higher yields for
the first harvest across the state. Butlerville maintained an
adequate amount of soil moisture for the entire growing
season. Lack of mid-summer moisture and excessive heat
limited growth at West Lafayette, while Wanatah and Columbia
City were less affected. Late-summer moisture fell statewide,
permitting alfalfa to enter the winter in good shape.
Early season rain in 1996 made it difficult to establish
alfalfa stands as well as delaying most of the first cutting
across the state. Due to this delay, the two northern sites
only reported three harvests for the year. Lack of moisture
at West Lafayette caused a decrease in yield toward the end
of the season. The study seeded in 1993 at Butlerville had to
be abandoned this spring due to heaving in excess of six
inches. Plentiful rainfall at Butlerville provided for above
average yields on the 1995 seeded study. 

Participating seed companies selected entries to be
tested. Seed was sent to Purdue University for planting and
evaluation. Beginning in 1994, commercial entries were
obtained through a seed procurement program initiated by
recommendation of the North American Alfalfa Improvement
Conference. Experimental entries (i.e. experimental
generations) from companies sent to Purdue were accepted into
yield tests, with data to be clearly marked as from a
non-commercial entry.
Between 1993 and 1996, Purdue University successfully
established ten alfalfa performance trials at four locations
across Indiana. Test plots were seeded into conventionally
prepared seedbeds. Benefin (Balan) herbicide was incorporated
into the soil prior to seeding. Seed was inoculated with
Rhizobium bacteria and treated with metalaxyl (Apron)
fungicide. Plots were seeded with a five-row press-wheel
seeder with 6-inch row spacings.
Best management practices were administered to all
studies. Optimum pH and fertility were provided and
maintained. Alfalfa weevil and potato leafhopper were
controlled with the systemic insecticides carbofuran
(Furadan) and dimethoate (Cygon), respectively. When
necessary, control of broadleaf or grass weeds was
accomplished with application of 2,4D-B (Butyrac), sethoxydim
(Poast) and imazethapyr (Pursuit) herbicides, respectively.
Winter-annual weeds were controlled on new and established
stands with an application of pronamide (Kerb) and metribuzin
(Sencor) herbicides, respectively. A flail-type forage
harvester was used to harvest plots, generally in late-bud to
early-flower stage. Hand samples were utilized for dry matter
determination. 

Yields are reported as dry matter yield in tons per acre
(T/A). Tables 1-9 summarize results of 1993 - 1996 alfalfa
variety yield trials conducted in Indiana.
In each table, varieties are listed in order of total
yield to date. Within a column, varieties differing from each
other by less than the respective LSD (least significant
difference) were not significantly (probability > 0.05)
different. Yields followed by an asterisk (*) are not
significantly different from the highest value in the column.
The CV (coefficient of variability) is the ratio of the
standard deviation to the grand mean. It is used as a measure
of the precision of the experiment. Lower CV's indicate lower
experimental error in the trial.
Number of harvests within a year is listed at the bottom
of each yield column.
Yield as percent of check is listed in the rightmost
columns of Tables 1-9. The check variety used was Vernal.
Additionally, Tables 1 and 5 show percent of check in the
first two years and in the final two years of production.
This can be used as a measure of persistence. Percentages
that increase with time or are relatively high in the final
years may be an indication of better persistence.
In 1994, the North American Alfalfa Improvement Conference
recommended new guidelines to separate entries allocated from
commercial and experimental seed sources. In the presentation
of the 1994-1996 seeded studies, names of entries are
preceded by "x" if tested using experimental seed
provided by the entrant; remaining entries were obtained from
commercial seed lots. Research has shown yield tends to
decrease in some breeding lines as seed progresses from a
more heterozygous state in experimental generations to the
commercially available generation.
Appendix Table 3 contains a listing of commercially
available entries, reference number of their marketer(s) in
correspondence with Appendix Table 4, tables where data is
found, and characterization information including fall
dormancy rating and resistance rating to bacterial wilt,
verticillium wilt, fusarium wilt, anthracnose, phytophthora
root rot, stem nematode, and root-knot nematode.
Appendix Table 4 lists marketer, address, phone number,
and contact person as provided by the entrant company and
referenced in Appendix Table 3. 

The following is information about each location.
The Pinney Purdue Agricultural Center is located near
Wanatah in Porter and LaPorte Counties (Jon D. Leuck,
superintendent). Studies seeded in 1994 and 1995 (Tables 2
and 3) are on a Sebawa loam soil on a 0-2% slope, formed in
loamy glacial outwash. The 1993 seeded study (Table 1) is on
a Tracy sandy loam on a 0-2% slope and was irrigated when
needed.
The Northeast Purdue Agricultural Center is located near
Columbia City in Whitley County (Philip C. Walker,
superintendent). The 1994 (Table 4) wes conducted on a Rawson
sandy loam on a 2-6% slope.
The Purdue Agronomy Research Center is located near West
Lafayette in Tippecanoe County (James J. Beaty III,
superintendent). The 1993 study (Table 5) was conducted on a
Chalmers silt loam (0-2 % slope). A Drummer silt loam with a
0-2% slope and a 4-5% organic matter content is the site of
the 1994 and 1995 seeded trials (Tables 6 and 7). Both are
highly-productive, prairie-derived soils. The 1996 seeded
study (Table 8) was conducted on a Xenia silt loam soil with
a 0-2% slope.
The Southeast Purdue Agricultural Center is located near
Butlerville in Jennings County (Donald J. Biehle,
superintendent). The 1995 seeded study (Table 9) is on an
Avonburg silt loam soil with a 0-2% slope. 

Appendix Table 1. Hay Statistics for Indiana and the
United States, 1993 to 19961.